| Study on the entry of recombinant HMGN2 protein into osteosarcoma cells and cell localization |
| Received:August 29, 2019 Revised:December 17, 2019 Click here to download the full text |
| Citation of this paper:MA Xiao,XU En-jie,YIN Jia,ZHOU Xu-hui.Study on the entry of recombinant HMGN2 protein into osteosarcoma cells and cell localization[J].Chinese Journal of Clinical Medicine,2020,27(2):308-312 |
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| Author Name | Affiliation | E-mail | | MA Xiao | Department of Spine Surgery, Changzheng Hospital, Naval Medical University, Shanghai 200003, China | | | XU En-jie | Department of Spine Surgery, Changzheng Hospital, Naval Medical University, Shanghai 200003, China | | | YIN Jia | Department of Spine Surgery, Changzheng Hospital, Naval Medical University, Shanghai 200003, China | | | ZHOU Xu-hui | Department of Spine Surgery, Changzheng Hospital, Naval Medical University, Shanghai 200003, China | xhzhouspine@163.com |
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| Abstract:Objective: To prepare high mobility group chromosal protein N2 (HMGN2) protein in vitro, and observe its capacity to enter into osteosarcoma cells and its cell localizations. Methods: After packaging HMGN2 overexpressing lentivirus, the recombinant lentivirus was delivered into HEK-293FT cells. HMGN2 protein was purified from the harvested 293FT cells using protein purification kit. Purified HMGN2 protein was used to treat cultured osteosarcoma cells. Whether the exogenous HMGN2 could enter into osteosarcoma cells and its cell localizations were detected by immunocytochemistry assay. Results: HMGN2 protein was successfully prepared and purified. The exogenous HMGN2 protein entered into osteosarcoma cells in culture, and mainly located in the nucleus. Conclusions: HMGN2 could be successfully prepared and purified, and can enter into cells. It mainly localizes in the nucleus, to lay a foundation for its biological effects. |
| keywords:osteosarcoma metastasis high mobility group chromosal protein N2 protein purification |
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