| Diagnostic value of extra-pulmonary tuberculosis by fine needle aspiration cytology combined with qPCR for extrapulmonary tuberculosis |
| Received:June 10, 2019 Revised:July 29, 2019 Click here to download the full text |
| Citation of this paper:WANG Li,HAO Ying-hua,LIU Ping,CHENG Xiu-weng,TANG Xian-bin.Diagnostic value of extra-pulmonary tuberculosis by fine needle aspiration cytology combined with qPCR for extrapulmonary tuberculosis[J].Chinese Journal of Clinical Medicine,2020,27(2):317-320 |
| Hits: 1847 |
| Download times: 1493 |
| Author Name | Affiliation | E-mail | | WANG Li | Department of Pathology, Taihe Hospital, Hubei University of Medicine, Shiyan 442000, Hubei, China | | | HAO Ying-hua | Department of Pathology, Taihe Hospital, Hubei University of Medicine, Shiyan 442000, Hubei, China | | | LIU Ping | Department of Pathology, Taihe Hospital, Hubei University of Medicine, Shiyan 442000, Hubei, China | | | CHENG Xiu-weng | Department of Pathology, Taihe Hospital, Hubei University of Medicine, Shiyan 442000, Hubei, China | | | TANG Xian-bin | Department of Pathology, Taihe Hospital, Hubei University of Medicine, Shiyan 442000, Hubei, China | taihecexu@163.com |
|
| Abstract:Objective: To explore the diagnostic value of fine needle aspiration cytology (FNAC) combined with fluorescence quantitative polymerase chain reaction (qPCR) for suspected extrapulmonary tuberculosis. Methods: A total of 57 patients probably infected by mycobacterium tuberculosis (TB) or needed to be excluded from infected TB were selected, and their puncture fluid of body surface cysts were collected. DNA was extracted from the puncture fluid and the qPCR method was used to detect the DNA of TB. The results of qPCR detection, FNAC, and clinical diagnosis were compared. Results: Based on clinical diagnosis (N=23), the diagnostic coincidence rate of FNAC was 82.61% (19/23), and the coincidence rate of qPCR test was 86.96% (20/23). The diagnostic coincidence of the combination of two methods was 92.98% (22/23). Conclusions: Fine needle aspiration cytology combined with qPCR can effectively diagnose the extrapulmonary tuberculosis, and the diagnosis rate is higher than single method. |
| keywords:fine needle aspiration cytology fluorescence quantitative polymerase chain reaction extrapulmonary tuberculosis |
| HTML View Full Text View/Add Comment Download reader |
|
|
|