| Exosomes derived from dendritic cells post myocardial infarction caused dyslipidemia via mTOR-SREBP pathway |
| Received:April 12, 2019 Revised:May 15, 2019 Click here to download the full text |
| Citation of this paper:Bai Yingnan,Li Zhixing,Zhang Yaqi,Gao Wei,Shao Wenqi,Wang xiaoyan,Liu Haibo,Zhang Zhenzhong,Qian Sanli,Li Bingyu,Li Zhuoquan,Zou Yunzeng,Qian juying,Ge Junbo.Exosomes derived from dendritic cells post myocardial infarction caused dyslipidemia via mTOR-SREBP pathway[J].Chinese Journal of Clinical Medicine,2019,26(3):445-449 |
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| Author Name | Affiliation | E-mail | | Bai Yingnan | Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai, China | bai.yingnan@zs-hospital.sh.cn | | Li Zhixing | Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai, China | | | Zhang Yaqi | Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai, China | | | Gao Wei | Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai, China | | | Shao Wenqi | Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai, China | | | Wang xiaoyan | Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai, China | | | Liu Haibo | Shanghai East Hospital an affiliate to Tongji University Medical School, Shanghai, China | | | Zhang Zhenzhong | Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai, China | | | Qian Sanli | Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai, China | | | Li Bingyu | Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai, China | | | Li Zhuoquan | School of Medicine, Tongji University, Shanghai, China | | | Zou Yunzeng | Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai, China | | | Qian juying | Department of Cardiology, Zhongshan Hospital, Fudan University, Shanghai Institute of Cardiovascular Diseases, Shanghai, China 200032 | | | Ge Junbo* | Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai, China | ge.junbo1@zs-hospital.sh.cn |
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| Abstract:Objective: Dendritic cells (DCs), as important inflammatory cells, are involved in atherosclerosis. Exosomes secreted by DCs (DEXs) have raised abundant attention recently while little is known on the role in dyslipidemia post myocardial infarction. SREBP is an important transcription factor that involves in the lipogenesis through mTOR-SREBP signaling. The pathway inside hepatocytes could be altered by inflammatory signals. We hypothesized that DEXs post myocardial infarction alter lipid metabolism through the mTOR-SREBP pathway. Methods: DCs from mice were stimulated with the supernatants of normal cardiomyocytes (Control-DEX group), or necrotic cardiomyocytes (MI-DEX group). The hepatocytes were treated with the DEXs secreted from the two groups, and the synthesis and secretion of lipids were determined by the enzyme-labeling measuring instrument. The lipid profiles were detected in cell medium. The expression of mTOR-SREBP pathway was measured by Western Blot and RT-PCR. Results: After the intervention of MI-DEX，the level of cholesterol and LDL-c of hepatocytes were enhanced significantly and the expression of mTOR-SREBP increased. With SREBP inhibitor, the functions of synthesis and secretion of lipids declined with decreased expression of mTOR-SREBP. Conclusion: Exosomes derived from dendritic cells may play an important role in the dyslipidemia after myocardial infarction by disturbing mTOR-SREBP pathway in the hepatocytes. |
| keywords:dendritic cell exosome dyslipidemia myocardial infarction mTOR-SREBP pathway |
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