Effect of pioglitazone on liver microsome cytochrome P450 in diabetic rats |
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Citation of this paper:CHANG Wei1△, MIN Zhi-hui2△, CHENG Yun-feng2, TANG Kou-ming1*.Effect of pioglitazone on liver microsome cytochrome P450 in diabetic rats[J].Chinese Journal of Clinical Medicine,2019,26(1):84-88 |
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Author Name | Affiliation | CHANG Wei1△, MIN Zhi-hui2△, CHENG Yun-feng2, TANG Kou-ming1* | 1.Department of Clinical Pharmacy, Qingpu Branch, Zhongshan Hospital, Fudan University, Shanghai 201700, China
2.Institute of Clinial Science, Zhongshan Hospital, Fudan University, Shanghai 200032, China |
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Abstract:Objective:To explore the effects of pioglitazone on the cytochrome P450 in the liver microsomes of the diabetic rats. Methods:Forty rats were randomly divided into four groups (n=10 in each group): normal control group, diabetes model group, 3 mg/kg and 6 mg/kg pioglitazone groups. Except the normal control, all other groups were fed with high-fat diet for 4 weeks, and then diabetic model rats were established by intraperitoneal injection with streptozotocin (STZ) of 50 mg/kg once. After modeling, rats in the pioglitazone groups were continuously intragastric administrated with pioglitazone for 2 weeks, and then sacrificed and the liver microsomes were extracted for the assay of P450. Meanwhile, the blood glucose and body weight were measured. Results:Compared with the normal control, the blood glucose in the diabetes model group increased significantly (P<0.01), the expression of cytochrome P450 also significantly increased (P<0.05), and body weight significantly decreased (P<0.01). Compared with the diabetes model group, during the 2 weeks of gavage, with the increasing of pioglitazone dose, the blood glucose in pioglitazone groups decreased remarkably (P<0.01), and the expression of cytochrome P450 obviously decreased in the two pioglitazone groups (P<0.01), whereas the body weight increased (P<0.05). In addition, the correlation analysis showed that there was a significantly positive correlation between cytochrome P450 and blood glucose in rats and the correlation coefficient was 0.598 (P<0.01). There was no significant correlation between cytochrome P450 and body weight in rats. Conclusions:Pioglitazone could inhibit the activity of cytochrome P450 in the liver of diabetic rats, reduce the blood glucose, and increase body weight in diabetic rats. |
keywords:pioglitazone diabetes mellitus cytochrome P450 microsomes |
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