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| Citation of this paper:.[J].Chinese Journal of Clinical Medicine,2016,23(6):739-743 |
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| Abstract:Objective：To investigate the role of microRNAs in Fox M1 induced non small cell lung cancer (NSCLC) epithelial mesenchymal transition (EMT).
Methods：Over expression Fox M1 plasmid, Fox M1 shRNA, miR 539, miR 485 5p mimics and inhibitor were transfected into NSCLC cells. The expression of Fox M1 protein and mRNA, miR 539 and miR 485 5p were detected by Western blotting and real time PCR. The CCK 8 and cell migration was used to observe the effect of Fox M1, miR 539 and miR 485 5p on the proliferation and invasion of NSCLC. Luciferase reporter assay was used to explore the relationship between miRNA( miR 539 and miR 485 5p) and EMT regulatory protein (ZEB1 and Snail1).
Results：Real time RT PCR and Western blotting showed Fox M1 over expression inhibited the expression of miR 539, miR 485 5p, miR 539 and miR 485 5p in NSCLC cells were increased after transfected Fox M1 shRNA. MiR 539 and miR 485 5p suppressed enhancement of proliferation and invasion of NSCLC cells by Fox M1. miR 539 targeted and inhibited the translation of ZEB1,miR 485 5p targeted and inhibited the translation of Snail1 in NSCLC cells.
Conclusions：The mechanism of Fox M1 up regulation of EMT protein is to decrease the expression of miR 539 and miR 485 5p in NSCLC cells, thus to affect the proliferation and invasion of NSCLC cells, and to inhibit distant metastasis. |
| keywords:forkhead box M1 epithelial mesenchymal transition NSCLC microRNA |
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