| Propofol Induces Neurotoxicity by Inhibiting tPA Release of Neuronal Synapse in Mouse Hippocampus |
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| Citation of this paper:LIANG Chao,CANG Jing,XUE Zhanggang.Propofol Induces Neurotoxicity by Inhibiting tPA Release of Neuronal Synapse in Mouse Hippocampus[J].Chinese Journal of Clinical Medicine,2016,23(1):67-70 |
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| Author Name | Affiliation | E-mail | | LIANG Chao | Department of Anesthesiology, Zhongshan Hospital, Fudan University, Shanghai 200032, China | superwm226@aliyun.com | | CANG Jing | Department of Anesthesiology, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | XUE Zhanggang | Department of Anesthesiology, Zhongshan Hospital, Fudan University, Shanghai 200032, China | |
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| Abstract:Objective: To explore the effect of propofol on the tPA release of hippocampal neurons in developing mice, so as to investigate whether exogenous tPA could reverse the propofol-induced neurotoxicity on developing neurons. Methods: The hippocampal neurons of developing mice cultured in vitro were divided into 4 groups(n=20 each):control group(group C),propofol group(group Pro), group tPA and propofol plus tPA group(group Pro+tPA).There was no specific treatment in group C. In group Pro,propofol was added to the culture media with the final concentration of 5,0, 30 μmol/L, respectively, and the cells were then incubated for 1 h, 2 h, 3 h, 6 h, respectively.In group tPA,tPA was added to the culture media with the final concentration of 1 μmol/L,and the cells were then incubated for 6 h.In group Pro+tPA, both propofol and tPA were added to the culture with the final concentration of 10 μmol/L and 1 μmol/L,respectively, and the cells were then incubated for 6 h.The neuron apoptosis rate and the expression level of actived-caspase-3 protein were detected in each group. Results: Compared with that in group C,the apoptosis rate and the expression level of actived-caspase-3 protein in group Pro significantly increased (P＜0.05).The tPA level in group Pro was significantly higher than that in group C(P＜0.05). Compared with that in group Pro,the apoptosis rate and the expression level of actived-caspase-3 protein in group Pro+tPA significantly decreased (P＜0.05). Conclusions: Propofol induces neurotoxicity on hippocampal neurons of developing mice cultured in vitro by inhibiting neuronal tPA release. And exogenous tPA could partially reverse propofol-induced neurotoxicity. |
| keywords:Propofol Developing neurons Neurotoxicity |
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