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胶质瘤发病相关蛋白1基因对香烟烟雾暴露小鼠肺部菌群的影响

Effects of GLI pathogenesis-related 1 gene on lung microbiome of mice exposed to cigarette smoke

    摘要
  • 摘要:
    目的 探讨胶质瘤发病相关蛋白1(GLI pathogenesis-related 1, GLIPR1)基因对香烟烟雾暴露小鼠的肺部菌群的影响。
    方法 通过被动吸入二手烟构建香烟烟雾暴露COPD小鼠模型, 将C57BL/6小鼠和GLIPR1基因敲除小鼠随机分为野生对照组(WT组)、野生型香烟烟雾暴露组(WT_SMOKE组)、GLIPR1基因敲除对照组(GLIPR1组)和GLIPR1基因敲除香烟烟雾暴露组(GLIPR1_SMOKE组)。采用16S rRNA测序技术得出肺部菌群丰度及多样性信息; 采用LEfSe分析各组小鼠肺部菌群的差异。
    结果 4组小鼠肺部微生物群β多样性差异显著(PANOSIM=0.003), 4组小鼠肺部微生物群间存在28个差异菌属。进一步LEfSe分析提示, 与WT组相比, WT_SMOKE组布劳特氏菌占比减少0.25%线性判别式分析(LDA)评分=3.1367 log10, P=0.006。GLIPR1_SMOKE组双歧杆菌属(LDA评分=3.465 log10, P=0.042)和芽单胞杆菌门S0134_terrestrial_group(LDA评分=3.115 log10, P=0.041)丰度最高。
    结论 香烟烟雾暴露后, GLIPR1基因敲除小鼠的肺部菌群分布与野生型小鼠存在差异, 其中以布劳特氏菌、双歧杆菌属和芽单胞杆菌门S0134_terrestrial_group差异最明显。

     

    Abstract:
    Objective To explore the effect of GLI pathogenesis-related 1(GLIPR1) gene on the lung microbiome of mice exposed to cigarette smoke.
    Methods Mice were exposed to cigarette smoke to established COPD mouse model.C57BL/6 mice and GLIPR1 konckout mice were randomly divided into 4 groups: wild-type control group (WT group), wild-type cigarette smoke exposure group (WT_SMOKE group), GLIPR1 gene knockout control group (GLIPR1 group), and GLIPR1 gene knockout cigarette smoke exposure group (GLIPR1_SMOKE group).Then 16S rRNA sequencing technology was used to analyze the differences among the lung microbiome of mice in 4 groups.
    Results There were significant differences in β diversity among the lung microbiota of 4 groups (PANOSIM=0.003).At the genus level, there were 28 differential bacterial genera among the 4 groups.LEfSe analysis showed that compared with WT group, the proportion of Blautia in mice of WT_SMOKE group decreased by 0.25%(LDA score=3.137 log10, P=0.006).The proportion of Bifidobacterium(LDA score=3.465 log10, P=0.042) and Gemmatimonadetes.S0134_terrestrial_group(LDA score=3.115 log10, P=0.041) were highest in GLIPR1_SMOKE group, respectively.
    Conclusions After exposure to cigarette smoke, the distribution of lung microbiome in GLIPR1 gene knockout mice is higher than that in WT mice, and Blautia, Bifidobacterium and Gemmatimonadetes.S0134_terrestrial_group play important roles.

     

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