摘要: |
目的 探讨肝细胞肝癌(hepatocelluar carcinoma,HCC)中c-Met、Fascin及CD44的表达与临床病理特征及预后之间的关系。方法 从癌症基因组图谱(the cancer genome atlas,TCGA)数据库下载HCC中c-Met、Fascin及CD44的RNA SEqV2资料,比较50例HCC组织和对应癌旁肝组织中三者的表达。采用蛋白质印迹法分别检测6例新鲜HCC及对应癌旁肝组织中c-Met、Fascin及CD44的蛋白表达。通过免疫组织化学法(immunohistochemistry,IHC)检测186例HCC患者中HCC组织及癌旁组织c-Met、Fascin及CD44蛋白的表达,分析三者与临床病理特征及预后的关系。结果 TCGA显示,HCC组织中c-Met、Fascin及CD44的mRNA表达较癌旁肝组织升高(P<0.01)。蛋白质印迹显示,新鲜HCC组织中c-Met、Fascin及CD44蛋白表达水平均高于癌旁肝组织(P<0.05)。IHC显示,186例HCC患者癌组织及癌旁组织中,c-Met蛋白表达阳性率分别为60.2%(112/186)和32.8%(61/186),Fascin蛋白表达阳性率分别为56.5%(105/186)和24.7%(46/186),CD44蛋白表达阳性率分别为73.1%(136/186)和50.5%(94/186),3种蛋白在HCC组织中的表达均高于癌旁组织(P<0.01)。c-Met、Fascin及CD44蛋白在脉管侵犯、低分化及术后复发患者癌组织表达中均增加(P<0.05)。Spearman相关性分析显示,c-Met与Fascin的表达正相关(r=0.349 5,P<0.001)。Kaplan-Meier生存分析及多因素Cox回归分析显示,c-Met、Fascin及CD44表达是影响HCC患者生存的危险因素(P<0.05)。结论 c-Met、Fascin及CD44的mRNA和蛋白表达水平在HCC组织中均升高,在脉管侵犯、肿瘤低分化及复发患者HCC组织中水平更高,且为HCC患者生存的独立危险因素。 |
关键词: c-Met Fascin CD44 肝细胞肝癌 脉管癌栓 复发 |
DOI:10.12025/j.issn.1008-6358.2022.20220599 |
分类号:R735.7 |
基金项目:南通市市级科技计划项目(MSZ21037),南通市卫生健康委员会科研课题专项(MB2021058,MA2019010,MB2020031). |
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Associations of c-Met, Fascin and CD44 with clinical pathological features and prognosis of patients with hepatocellular carcinoma |
ZHU Lin1, CHEN Li2, XIAO Feng1, ZHANG Hai-yan1, GU Chun-yan1
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1.Department of Pathology, Nantong 3rd People's Hospital, Nantong Third Hospital Affiliated Nantong University, Nantong 226000, Jiangsu, China;2.Department of Pathology, Nantong University Medical School, Nantong 226001, Jiangsu, China
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Abstract: |
Objective To explore the association of c-Met, Fascin and CD44 with the clinical pathological fetures and prognosis of patients with hepatocellular carcinoma (HCC). Methods The RNA SEqV2 data of c-Met, Fascin and CD44 in HCC from the cancer genome atlas (TCGA) were downloaded (n=50). The mRNA levels of c-Met, Fascin and CD44 between cancer and paracancer tissues were compared. Western blotting was used to detect the expression levels of c-Met, Fascin and CD44 proteins in 6 cases of cancer and the matched paracancer liver tissues. The expression levels of c-Met, Fascin and CD44 proteins were examined in cancer and paracancer liver tissues from 186 patients by immunohistochemistry (IHC). The associations of c-Met, Fascin and CD44 with clinical pathological factors and prognosis of patients were analyzed. Results The data from TCGA showed that the mRNA levels of c-Met, Fascin and CD44 were significantly higher in cancer tissues than those in the paracancer tissues (P<0.01). Western blotting results showed that the protein levels of c-Met, Fascin and CD44 in fresh cancer tissues were significantly higher than those in the paracancer tissues (P<0. 05). IHC results showed the c-Met expressed in 60.2% (112/186) of cancer tissues and 32.8% (61/186) of paracancer tissues, Fascin expressed in 56.5% (105/186) of cancer tissues and 24.7% (46/186) of paracancer tissues, and CD44 expressed in 73.1% (136/186) of cancer tissues and 50.5% (94/186) of paracancer tissues. The protein levels of c-Met, Fascin and CD44 in cancer tissues were higher than those in paracancer tissues (P<0.01). The expressions of c-Met,Fascin and CD44 proteins in patients with vascular invasion or poor differentiation tumor and in recurrence patients were higher (P<0.05). Spearman analysis showed that there was a positive correlation between c-Met and Fascin (r=0.349 5, P<0.001). Kaplan-Meier and multivariate Cox analysises all showed that higher levels of c-Met, Fascin and CD44 were risk factors for HCC patients survival (P<0.05). Conclusion c-Met, Fascin and CD44 expressions are increased in HCC cancer tissues, are higher in vascular invasion, lower tumor differentiation, and recurrence patients, and may be independent risk factors for HCC prognosis. |
Key words: c-Met Fascin CD44 hepatocellular carcinoma vascular invasion relapse |