摘要: |
目的:探讨抑郁、焦虑状态人群与健康人群肠道菌群组成的差异。方法:选择2017年5月至2018年4月复旦大学附属中山医院全科和心理门诊收治的237例就诊者,根据抑郁症筛查量表(patien health questionnare,PHQ-9)和广泛性焦虑障碍量表(generalized anxiexy disorde-7,GAD-7)的评估,分为单纯抑郁组(n=38)、单纯焦虑组(n=36)和抑郁合并焦虑组(n=43)和健康对照组(n=120)。收集4组人群的粪便样本,采用高通量测序及生物信息的方法,分析菌群构成的多样性和不同层面的菌群丰度,比较组间的肠道菌群构成差异。结果:单纯抑郁组、单纯焦虑组、抑郁合并焦虑组与健康对照组相比,α和β多样性差异无统计学意义。在属的层面上,单纯抑郁组和单纯焦虑组中梭菌属(Clostridium)的丰度显著降低。结论:抑郁、焦虑状态人群与健康人群的肠道菌群结构未发现显著差异,梭菌属可能在抑郁、焦虑的发展中发挥着一定作用。 |
关键词: 肠道菌群 抑郁 焦虑 16S rRNA测序 |
DOI:10.12025/j.issn.1008-6358.2021.20202551 |
分类号:R378.2 |
基金项目:上海市科学技术委员会科研计划项目(17ZR1404900),上海市医学领军人才项目(2019LJ15). |
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Composition of intestinal flora in people with depression and anxiety |
MO Han-jun1, LANG Lin1, LIU Li-na1, LAO Li-min1, JIANG Sun-fang1,2
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1.Department of General Practice, Zhongshan Hospital, Fudan University, Shanghai 200032, China;2.Health Management Center, Zhongshan Hospital, Fudan University, Shanghai 200032, China
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Abstract: |
Objective: To explore the difference in gut microbiota composition between patients with depressive or anxiety state and healthy people. Methods: From May 2017 to April 2018, 237 patients of department of general practice and psychological clinic of Zhongshan Hospital, Fudan University were selected. According to the evaluation of patient health questionnaire (PHQ-9) and generalized anxiety disorder-7 (GAD-7), they were divided into simple depression group (n=38), simple anxiety group (n=36), depression combined with anxiety group (n=43) and healthy control group (n=120). Feces of patients were collected. High-throughput sequencing and bioinformatics were used to analyze diversity and abundance at different levels of gut microbiota. Results: Compared with the healthy controls, there was no significant difference in alpha and beta diversity in the simple depression group, simple anxiety group, and depression combined with anxiety group. At the genus level, the abundance of Clostridium significantly reduced in the simple depression group and simple anxiety group. Conclusions: There is no significant difference in the structure of gut microbiota between patients with depressive or anxiety state and healthy people. Clostridium may play a role in the development of depression and anxiety. |
Key words: gut microbiota depression anxiety 16S rRNA sequencing |