摘要: |
目的:探讨PPARγ激动剂罗格列酮与慢性阻塞性肺疾病(COPD)大鼠的血管重塑、炎症指标白介素-8(IL-8)的相关性。方法:36只Wistar大鼠随机分为非药物治疗组(A组)、药物治疗组(B组)、空白对照组(C组),A、B组用烟熏、低氧、脂多糖(LPS)的方法建立COPD大鼠模型;B组第3周起同时给予盐酸罗格列酮药物治疗,C组给予相同体积的生理盐水模拟造模和治疗。结果:血管壁病理示B组血管壁重塑、胶原蛋白的沉积及血管周围中性粒细胞浸润情况较A组减轻,但仍较C组严重。A组、B组、C组之间肺血管壁/肺血管总面积(MA%)差异具有统计学意义(P<0.01),A组MA%较C组明显升高,B组MA%较A组明显下降。炎性指标IL-8在3组之间差异具有统计学意义(P<0.01),其中A组明显高于B组,B组高于C组。3组的肺组织中PPARγ转录水平差异具有统计学意义(P<0.01),C组、B组、A组肺组织中PPARγ转录水平逐渐下降,其中A组明显高于B组,B组高于C组。Pearson分析显示B组大鼠中PPARγ转录水平与MA%比值、炎症指标IL-8均负相关(r=-0.662、-0.615;P<0.05),而B组中MA%与IL-8正相关(r=0.980,P<0.05)。结论:PPARγ激动剂罗格列酮通过上调PPARγ转录水平抑制COPD大鼠肺部IL-8的升高和血管周围中性粒细胞聚集、抑制了肺血管壁的重塑。 |
关键词: 肺性 慢性阻塞性 血管重塑 炎症 PPARγ IL-8 |
DOI:10.12025/j.issn.1008-6358.2020.20191756 |
分类号:R56.3;R543.2 |
基金项目:山东省医药卫生科技发展计划项目(2009-H2028). |
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Effects of PPARγ agonists on inflammation and vascular remodeling in COPD rats |
WANG Chen, LI Zhi, TAN Lin
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Intensive Care Unit, Qingdao Municipal Hospital, Qingdao University, Qingdao 266071, Shandong, China
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Abstract: |
Objective: To explore the effects of rosiglitazone hydrochloride on vascular remodeling and IL-8 in rat models of chronic obstructive pulmonary disease(COPD).Methods: Thirty-six Wistar rats were randomized to three groups: non-treatment group (group A), treatment group (group B), and blank group (group C). Rat model of COPD was established in groups A and B by using smoking, hypoxia and intratracheal lipopolysaccharide. The rats in group B were treated with rosiglitazone from week 3, and those in group C were given identical dose of normal saline.Results: The vascular wall pathology showed that the vascular wall remodeling, collagen deposition, and perivascular neutrophils infiltration in group B were alleviated than in group A, but more serious than in group C. The ratio of pulmonary vascular wall/total vascular area in group A were higher than that in groups C and B (P<0.01). The level of IL-8 in group A were higher than in groups C and B (P<0.01). PPARγ mRNA in lung tissue increased in order of groups A, B, and C (P<0.01). Pearson analysis showed that in group B, the levels of PPARγ mRNA were negatively correlated with the ratio of pulmonary vascular wall/total vascular area and the level of IL-8 (r=-0.662 and -0.615, P<0.05).Conclusions: Rosigltazone inhibits the remodeling of the pulmonary vascular wall in COPD rats by upregulating transcription of PPARγ and inhibiting the increase of IL-8 and the infiltration of neutrophils around the vascular wall. |
Key words: pulmonary disease chronic obstructive vascular remodeling inflammation peroxisome proliferators-actived receptor γ IL-8 |