摘要: |
血管内皮细胞(endothelial cells, ECs)既往被认为在出生后血管新生中发挥主要作用。1997年Asahara等从外周血分离出表达CD34抗原的单个核细胞,即血管内皮祖细胞(endothelial progenitor cells,EPCs)。EPCs是一类具有促血管生成能力的干细胞总称,尚未发现特异性免疫表型,故主要根据具有形成血管的功能鉴定。目前一般认为,EPCs来源于骨髓和外周血管壁,其中能分化为ECs者被称为内皮集落形成细胞(endothelial colony forming cells,ECFCs)。当组织缺血或损伤时,来自骨髓的EPCs被组织释放的损伤信号动员后进行迁移和归巢。活化的EPCs通过旁分泌作用招募更多的局部ECFCs和ECs来形成微血管,进而促进组织修复。因此,对EPCs的深入研究有助于临床上治疗性血管新生相关研究的进行和方法的发展。 |
关键词: 血管内皮祖细胞 内皮集落形成细胞 治疗性血管新生 |
DOI:10.12025/j.issn.1008-6358.2019.20190351 |
分类号:R 783 |
基金项目:国家自然科学基金(81970407), 上海市优秀学术带头人(19XD1401200),上海市卫生计生系统优秀学科带头人培养计划(百人计划,2018BR40),复旦大学附属中山医院人才培养计划优秀骨干计划(2015ZSYXGG02). |
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Research progress on vascular endothelial progenitor cells and their mechanism of promoting angiogenesis |
潘天岳,刘 浩,符伟国,董智慧
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Abstract: |
Endothelial cells (ECs) were considered to play a main role in postnatal angiogenesis until CD34+ mononuclear cells were isolated from peripheral blood by Asahara et al in 1997, and these cells were named as putative endothelial progenitor cells (EPCs). EPCs are a group of stem cells with angiogenic function, and have no specific immunophenotype. Identification of EPCs is mainly based on their vasculogenic ability. It is widely recognized that EPCs originate either from bone marrow or peripheral vascular wall. The cells which can differentiate to immature endothelial cells in vitro are called endothelial colony forming cells (ECFCs). Signals release from ischemic tissues mobilize the EPCs from bone marrow and guide them to migrate and home to the ischemic sites. The active EPCs recruit more resident ECFCs and ECs to form neomicrovessels and then promote the tissues repair. More intensive studies on the nature of EPCs will contribute to the development of clinical therapeutic angiogenesis research and methods. |
Key words: endothelial progenitor cells endothelial colony forming cells therapeutic angiogenesis |