摘要: |
目的:检测喉癌、喉良性病变组织及声带息肉组织中HPV-DNA及P16蛋白的表达水平,探讨HPV感染与喉癌发生的相关性。方法:收集2011年5月至2015年7月就诊于复旦大学附属金山医院耳鼻咽喉-头颈外科的256例患者,其中喉鳞状细胞癌34例、喉良性病变(非声带息肉)15例、声带息肉207例,男性184例、女性72例,中位年龄56岁。采用实时荧光PCR法检测喉鳞状细胞癌组织和喉良性病变组织HPV-DNA水平。采用免疫组化法观察喉癌组织、喉良性病变及声带息肉中P16蛋白的表达。结果:实时荧光PCR检测发现34例喉鳞状细胞癌组织中HPV-DNA阳性率为8.8%(3/34),喉良性病变均为阴性(0/15),两组间HPV-DNA阳性率差异无统计学意义。免疫组化染色显示喉鳞状细胞癌、喉良性病变、声带息肉组织P16蛋白阳性表达率分别为32.4%(11/34)、46.7%(7/15)和47.8%(99/207),其中声带息肉组织P16蛋白阳性率高于喉癌组织,差异有统计学意义(P=0.046)。结论:喉癌组织HPV-DNA阳性率略高于喉良性病变;P16蛋白在喉癌组织的表达略低于喉良性病变,明显低于声带息肉。 |
关键词: 喉癌 喉良性病变 声带息肉 人乳头状瘤病毒(HPV) 实时荧光PCR |
DOI: |
分类号:R739.6 |
基金项目:上海市金山区科学技术创新基金项目(2014-3-20). Supported by Science and Technology Innovation Fund of Jinshan District of Shanghai(2014-3-20). |
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Abstract: |
Objective:To detect the expression of HPV-DNA and P16 protein in laryngeal cancer, laryngeal benign lesion tissue, and vocal cord polyp tissue, and to explore the correlation between HPV infection and laryngeal cancer. Methods:The clinical data of 256 patients who had been treated in Department of Otorhinolaryngology & Head and Neck Surgery of Jinshan Hospital, Fudan University from May 2011 to July 2015 were collected. There were 34 cases of laryngeal squamous cell carcinoma, 15 cases of benign laryngeal lesions (non-vocal cord polyps), 207 cases of vocal cord polyps, 184 males and 72 females, with a median age of 56 years. The levels of HPV-DNA in laryngeal squamous cell carcinoma tissues and benign laryngeal lesions were detected by real-time fluorescence PCR. Immunohistochemical staining was used to detect the expression of P16 protein in laryngeal cancer tissues, laryngeal benign lesions and vocal cord polyps. Results: Real time fluorescence PCR detection showed that the HPV-DNA positive rate in 34 cases of laryngeal squamous cell carcinoma was 8.8% (3/34), and it was all negative (0/15) in benign laryngeal lesions. There was no significant difference in the HPV-DNA positive rate between the two groups. Immunohistochemical staining showed that the positive expression rate of P16 protein in laryngeal squamous cell carcinoma, benign laryngeal lesions and vocal cord polyp tissues was 32.4% (11/34), 46.7% (7/15), and 47.8% (99/207). The positive rate of P16 protein in vocal cord polyp tissues was higher than that of laryngeal cancer tissues, and the difference was statistically significant (P=0.046). Conclusions:The positive rate of HPV-DNA in laryngeal cancer tissues is slightly higher than that in benign laryngeal lesions. The expression of P16 protein in laryngeal cancer tissues is slightly lower than that in benign laryngeal lesions, and is significantly lower than that in vocal cord polyps. |
Key words: laryngeal cancer benign laryngeal lesions vocal cord polyp human papillomavirus (HPV) real-time fluorescence PCR |