Abstract: Objective：To investigate the expression of miRNA-29b in ovarian cancer cells and its effects on proliferation and invasion of ovarian cancer cells and the underlying mechanisms. Methods：Real-time RT-PCR was used to detect the expression level of miRNA-29b in human ovarian cancer epithelial cell line SKOV-3 and human normal ovarian epithelial cell line IOSE80. The expression of miRNA-29b in SKOV-3 cells was up-regulated by transfection technique. CCK-8 assay was used to detect the proliferation of SKOV-3 cells. Transwell migration assay was used to detect the invasion of SKOV-3 cells. Western blotting was used to detect the level of protein expression in SKOV-3 cells. Results：The expression of miRNA-29b in human ovarian epithelial cell line SKOV-3 cells decreased significantly (compared with IOSE80 cells, P<0.05). The expression level of miRNA-29b in SKOV-3 cells was increased significantly after miRNA-29b mimic transfection (P<0.05). From the 4th day, the proliferation of miRNA-29b-transfected SKOV3 cell was significantly increased (P<0.05). At the same time, transfection of miRNA-29b significantly reduced the number of SKOV3 cells through the polycarbonate membrane and inhibited the invasiveness of SKOV3 cells (P<0.05). After transfection of miRNA-29b mimic, the expression of Bcl-2 and the invasion-related protein MMP-9 in the ovarian cancer cell line SKOV3 were significantly lower than those in the negative control group (P<0.05). Conclusions：miRNA-29b can inhibit the proliferation and invasion of ovarian cancer cells, probably via inhibiting the expression of apoptosis related protein Bcl-2 and invasion related protein MMP-9.