LncRNA A-30-P01019163 regulates βB2 crystallin gene expression in ovarian granulosa cells and its effects on cell proliferation and apoptosis |
Received:March 05, 2021 Revised:May 06, 2021 Click here to download the full text |
Citation of this paper:LIU Sheng-nan,LI Mei-hui,LI Li,HUANG Wei,ZHANG Jun-jie.LncRNA A-30-P01019163 regulates βB2 crystallin gene expression in ovarian granulosa cells and its effects on cell proliferation and apoptosis[J].Chinese Journal of Clinical Medicine,2021,28(5):795-801 |
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Author Name | Affiliation | E-mail | LIU Sheng-nan | Department of Obstetrics and Gynecology, the First Affiliated Hospital of Naval Medical University, Shanghai 200433, China | | LI Mei-hui | Department of Obstetrics and Gynecology, the First Affiliated Hospital of Naval Medical University, Shanghai 200433, China | | LI Li | Department of Obstetrics and Gynecology, the First Affiliated Hospital of Naval Medical University, Shanghai 200433, China | | HUANG Wei | Department of Obstetrics and Gynecology, the First Affiliated Hospital of Naval Medical University, Shanghai 200433, China | | ZHANG Jun-jie | Department of Obstetrics and Gynecology, the First Affiliated Hospital of Naval Medical University, Shanghai 200433, China | zhangjj910@163.com |
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Abstract:Objective: To explore the regulatory effect of lncRNA A-30-P01019163 on the expression of ovarian granulocytes βB2 crystallin (CryBB2) and its effects on ovarian granulocyte proliferation and apoptosis.Methods: The model of over-expression lncRNA A-30-P01019163 was constructed, and a stable cell line based on human ovarian granulosa cell line KGN with of overexpression or interference lncRNA A-30-P01019163 was established. The expression level of CryBB2 in after expression or interference was detected by qRT-PCR and protein blotting method, and the cell proliferation was detected by EdU, and apoptosis was detected by TUNEL and flow cytometric analysis.Results: As the mice age increasesd, the expression of lncRNA A-30-P01019163 in the granulocytes gradually increasesd (P<0.05). After overexpression of lncRNA A-30-P01019163 in KGN cells, the CryBB2 mRNA and protein expressions increased (P<0.05); granulocyte proliferation accelerated, cell cycle-related protein D1 and proliferating cell nuclear antigen expressions increased (P<0.01); the apoptosis of granulocytes increased, and cleaved caspase-3 and poly ADP-ribose polymerase (PARP) expressions were all increased(P<0.05). After interfering lncRNA A-30-P01019163 in KGN cells, CryBB2 mRNA and protein expressions were reduced (P<0.01); cell proliferation slowed down, cell cycle-related protein D1 and proliferating cell nuclear antigen expressions decreased (P<0.01); cell apoptosis decreased, cleaved caspase-3 and PARP expressions decreased (P<0.05).Conclusions: The expression of lncRNA A-30-P01019163 increased along with ovarian granulosa cell development and is involved in the regulation of CryBB2 protein expression. LncRNA A-30-P01019163 is closely related to proliferation and apoptosis of ovarian granulosa cells. |
keywords:lncRNA βB2 crystallin ovarian granulosa cell proliferation apoptosis |
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