Quick Search:       Advanced Search
LncRNA A-30-P01019163 regulates βB2 crystallin gene expression in ovarian granulosa cells and its effects on cell proliferation and apoptosis
Received:March 05, 2021  Revised:May 06, 2021  Click here to download the full text
Citation of this paper:LIU Sheng-nan,LI Mei-hui,LI Li,HUANG Wei,ZHANG Jun-jie.LncRNA A-30-P01019163 regulates βB2 crystallin gene expression in ovarian granulosa cells and its effects on cell proliferation and apoptosis[J].Chinese Journal of Clinical Medicine,2021,28(5):795-801
Hits: 840
Download times: 440
Author NameAffiliationE-mail
LIU Sheng-nan Department of Obstetrics and Gynecology, the First Affiliated Hospital of Naval Medical University, Shanghai 200433, China  
LI Mei-hui Department of Obstetrics and Gynecology, the First Affiliated Hospital of Naval Medical University, Shanghai 200433, China  
LI Li Department of Obstetrics and Gynecology, the First Affiliated Hospital of Naval Medical University, Shanghai 200433, China  
HUANG Wei Department of Obstetrics and Gynecology, the First Affiliated Hospital of Naval Medical University, Shanghai 200433, China  
ZHANG Jun-jie Department of Obstetrics and Gynecology, the First Affiliated Hospital of Naval Medical University, Shanghai 200433, China zhangjj910@163.com 
Abstract:Objective: To explore the regulatory effect of lncRNA A-30-P01019163 on the expression of ovarian granulocytes βB2 crystallin (CryBB2) and its effects on ovarian granulocyte proliferation and apoptosis.Methods: The model of over-expression lncRNA A-30-P01019163 was constructed, and a stable cell line based on human ovarian granulosa cell line KGN with of overexpression or interference lncRNA A-30-P01019163 was established. The expression level of CryBB2 in after expression or interference was detected by qRT-PCR and protein blotting method, and the cell proliferation was detected by EdU, and apoptosis was detected by TUNEL and flow cytometric analysis.Results: As the mice age increasesd, the expression of lncRNA A-30-P01019163 in the granulocytes gradually increasesd (P<0.05). After overexpression of lncRNA A-30-P01019163 in KGN cells, the CryBB2 mRNA and protein expressions increased (P<0.05); granulocyte proliferation accelerated, cell cycle-related protein D1 and proliferating cell nuclear antigen expressions increased (P<0.01); the apoptosis of granulocytes increased, and cleaved caspase-3 and poly ADP-ribose polymerase (PARP) expressions were all increased(P<0.05). After interfering lncRNA A-30-P01019163 in KGN cells, CryBB2 mRNA and protein expressions were reduced (P<0.01); cell proliferation slowed down, cell cycle-related protein D1 and proliferating cell nuclear antigen expressions decreased (P<0.01); cell apoptosis decreased, cleaved caspase-3 and PARP expressions decreased (P<0.05).Conclusions: The expression of lncRNA A-30-P01019163 increased along with ovarian granulosa cell development and is involved in the regulation of CryBB2 protein expression. LncRNA A-30-P01019163 is closely related to proliferation and apoptosis of ovarian granulosa cells.
keywords:lncRNA  βB2 crystallin  ovarian granulosa cell  proliferation  apoptosis
HTML  View Full Text  View/Add Comment  Download reader