| PROX1 promoted the progression of non-small cell lung cancer via epithelial-mesenchymal transition |
| Received:March 03, 2021 Revised:June 26, 2021 Click here to download the full text |
| Citation of this paper:GAO Jian,AO Yong-qiang,ZHANG Ling-xian,WANG Shuai,DING Jian-yong,JIANG Jia-hao.PROX1 promoted the progression of non-small cell lung cancer via epithelial-mesenchymal transition[J].Chinese Journal of Clinical Medicine,2021,28(5):802-807 |
| Hits: 1148 |
| Download times: 272 |
| Author Name | Affiliation | E-mail | | GAO Jian | Department of Thoracic Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | AO Yong-qiang | Department of Thoracic Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | ZHANG Ling-xian | Department of Cardiothoracic Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi, China | | | WANG Shuai | Department of Thoracic Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China | | | DING Jian-yong | Department of Thoracic Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China | ding.jianyong@zs-hospital.sh.cn | | JIANG Jia-hao | Department of Thoracic Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China | jiang.jiahao@zs-hospital.sh.cn |
|
| Abstract:Objective: To explore the function and mechanism of PROX1 in non-small cell lung cancer (NSCLC). Methods: The tissue micro-array containing 108 NSCLC tissues was used to explore the relationship between the expression of PROX1 and the prognosis of NSCLC patients. Western blot and quantitative real-time polymerase chain reaction (qRT-PCR) were used to detect the expression of PROX1 in the NSCLC cell line and detect the biomarkers of epithelial-to-mesenchymal transition (EMT). Moreover, the CCK8 and Transwell assay was used to detect the proliferation, invasion, and migration ability of the NSCLC cell line. Results: The expression of PROX1 was upregulated in NSCLC. The expression of PROX1 was closely associated with tumor size and lymphocyte metastasis (P=0.003 and 0.042). The NSCLC patients in the PROX1 high group had a higher recurrence rate and lower survival rate than in the PROX1 low group with the five-year recurrence rate (70.9% vs 50.9%, P=0.005) and the five-year survival rate (49.1% vs 66%, P=0.016). The CCK-8 and Transwell assays showed that the proliferation, invasion, and migration of NSCLC were significantly reduced after the knockdown of PROX1(P<0.01、P<0.05). In terms of mechanism, the knockdown of PROX1 led to the upregulation of E-cadherin and reduction of vimentin(P<0.001). Conclusions: The upregulation of PROX1 contributes to the progression of NSCLC through EMT and it can be a potential biomarker for the prognosis of NSCLC. |
| keywords:Prospero-related homeobox 1 non-small cell lung cancer epithelial-mesenchymal transition prognosis |
| HTML View Full Text View/Add Comment Download reader |
|
|
|