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Remission effects of renalase on high glucose-induced injury in podocytes
Received:January 04, 2021  Revised:April 23, 2021  Click here to download the full text
Citation of this paper:ZHAO Ting,ZHANG Fang-fei,YIN Jian-yong,WANG Nian-song,WANG Feng.Remission effects of renalase on high glucose-induced injury in podocytes[J].Chinese Journal of Clinical Medicine,2021,28(4):603-609
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Author NameAffiliationE-mail
ZHAO Ting Department of Nephrology, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai 200030, China  
ZHANG Fang-fei Department of Nephrology, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai 200030, China  
YIN Jian-yong Department of Nephrology, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai 200030, China  
WANG Nian-song Department of Nephrology, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai 200030, China
Department of Nephrology, Shanghai Eighth People's Hospital, Shanghai 200235, China 
 
WANG Feng Department of Nephrology, Shanghai Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai 200030, China
Department of Nephrology, Shanghai Eighth People's Hospital, Shanghai 200235, China 
zyzwq1030@163.com 
Abstract:Objective: To explore the protective effects and underlying mechanism of renalase on high glucose-induced injury in podocytes. Methods: Mouse podocytes were cultured in vitro and divided into control group (5 mmol/L glucose), mannitol group (5 mmol/L glucose + 25 mmol/L D-mannitol), high glucose group (30 mmol/L glucose), and study group (60 μg/mL renalase for 6 hours + 30 mmol/L glucose). After cultured for 48 hours, cells were collected. The mRNA levels of collagen Ⅳα, transforming growth factor β1 (TGF-β1), and monocyte chemoattractant protein 1 (MCP-1) were detected by real-time fluorescence quantitative PCR (qRT-PCR). The activity of caspase-3 and cyclin-dependent kinase inhibitor 1 (p21) and levels of collagen Ⅳα, TGF-β1, MCP-1 were detected by enzyme-linked immunosorbent assay. Results: Compared with the control group, the levels of collagen Ⅳα,TGF-β1, and MCP-1 and the activity of caspase-3 and p21 were significantly increased in podocytes in the high glucose group (P<0.05). In contrast, the levels of collagen Ⅳα, TGF-β1, and MCP-1, as well as the activity of caspase-3 and p21, were decreased significantly in podocytes in the study group (P<0.05). Conclusions: Renalase could attenuate high glucose-induced injury in podocytes, of which the mechanism may be related to anti-inflammatory, anti-fibrotic, anti-apoptotic, and reducing p21 activity.
keywords:renalase  podocyte  high glucose
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