Clinical Observation Research on the Relationship Between Carbonic Anhydrase Ⅲ and Senile Type 2 Diabetes with Vascular Complications

Objective: To analyze the possible risk factors of senile diabetic angiopathy and explore the intrinsic correlation between CAIII and senile diabetic angiopathy. Methods: Screen the elderly population in the outpatient department of the Geriatrics Department of Huashan Hospital affiliated to Fudan University in 2017. The occurrence of vascular complications in patients with T2DM and the changes of serum biochemical indicators were observed. According to the inclusion criteria and exclusion criteria, they were divided into three groups: control group, T2DM without vascular complication group, T2DM with vascular complication group. In addition to routine physical examination items， the serum CAIII levels were detected by ELISA. Data were analyzed using SPSS 25.0 statistical software. Results: Compared with the control group, there were significant differences in ALT, TP, BUN, UA, TG, LDL, HDL, FBG and HbAlc in the T2DM group (P<0.05). The levels of TBIL in T2DM patients with vascular complications were significantly lower than that in T2DM patients without vascular complications (P<0.05). The results of ELISA showed that the level of CAIII in the T2DM without vascular complication group and T2DM with vascular complication group were significantly lower than that in the control group, while the level of CAIII in the T2DM group with vascular complication was lower than that in the T2DM group without vascular complication (P<0.01). Spearman correlation coefficient analysis showed that serum CAIII levels were positively correlated with HDL and TBIL, but there was no statistical difference; serum CAIII levels were negatively correlated with LDL, BUN, Scr, FBG and HbA1c, and there were statistical difference compared with BUN, FBG and HbA1c (P<0.05). The ROC curve analysis indicated that CAIII was higher accurate in diagnosing T2DM with vascular complication (P<0.05). Conclusion: Serum CAIII was closely related to the disorder of glucose and lipid metabolism，and vascular complications in elderly patients with T2DM, suggesting that CAIII may participate in the occurrence and development of elderly patients with diabetic angiopathy by regulating of glucose and lipid metabolism, but the specific underlying mechanisms need to be further studied in the future.