摘要: |
目的: 探讨转录因子Prospero同源框1(Prospero-related homeobox 1,PROX1)在非小细胞肺癌(non-small cell lung cancer,NSCLC)进展中的作用和机制。方法: 对含有108对非小细胞肺癌组织的组织芯片进行免疫组化染色,分析PROX1表达和NSCLC患者预后间的关系。采用Western印迹法和实时定量PCR分析PROX1在NSCLC细胞系中的表达情况和上皮间质转化的标志物。采用CCK-8、Transwell试验检测肿瘤细胞的增殖、迁移和侵袭能力。结果: PROX1在非小细胞肺癌组织中的表达上调。PROX1的表达与肿瘤直径、淋巴转移显著相关(P=0.003、0.042)。高表达PROX1的NSCLC患者其5年复发率高于PROX1低表达组(70.9%vs 50.9%,P=0.005),而5年生存率低于PROX1低表达组(49.1%vs 66.0%,P=0.016)。CCK-8和Transwell实验的结果显示,敲减PROX1后肿瘤的增殖、侵袭和转移能力明显减弱(P<0.01、P<0.05)。敲减PROX1后E-钙粘蛋白表达上调而波形蛋白表达下降(P<0.001)。结论: PROX1表达增高可通过促进上皮间质转化而促进NSCLC进展;PROX1是潜在的NSCLC预后预测因子。 |
关键词: 转录因子Prospero同源框1 非小细胞肺癌 上皮间质转化 预后 |
DOI:10.12025/j.issn.1008-6358.2021.20210443 |
分类号:R734.2 |
基金项目:国家自然科学基金(81972168). |
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PROX1 promoted the progression of non-small cell lung cancer via epithelial-mesenchymal transition |
GAO Jian1, AO Yong-qiang1, ZHANG Ling-xian2, WANG Shuai1, DING Jian-yong1, JIANG Jia-hao1
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1.Department of Thoracic Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China;2.Department of Cardiothoracic Surgery, The Second Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi, China
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Abstract: |
Objective: To explore the function and mechanism of PROX1 in non-small cell lung cancer (NSCLC). Methods: The tissue micro-array containing 108 NSCLC tissues was used to explore the relationship between the expression of PROX1 and the prognosis of NSCLC patients. Western blot and quantitative real-time polymerase chain reaction (qRT-PCR) were used to detect the expression of PROX1 in the NSCLC cell line and detect the biomarkers of epithelial-to-mesenchymal transition (EMT). Moreover, the CCK8 and Transwell assay was used to detect the proliferation, invasion, and migration ability of the NSCLC cell line. Results: The expression of PROX1 was upregulated in NSCLC. The expression of PROX1 was closely associated with tumor size and lymphocyte metastasis (P=0.003 and 0.042). The NSCLC patients in the PROX1 high group had a higher recurrence rate and lower survival rate than in the PROX1 low group with the five-year recurrence rate (70.9% vs 50.9%, P=0.005) and the five-year survival rate (49.1% vs 66%, P=0.016). The CCK-8 and Transwell assays showed that the proliferation, invasion, and migration of NSCLC were significantly reduced after the knockdown of PROX1(P<0.01、P<0.05). In terms of mechanism, the knockdown of PROX1 led to the upregulation of E-cadherin and reduction of vimentin(P<0.001). Conclusions: The upregulation of PROX1 contributes to the progression of NSCLC through EMT and it can be a potential biomarker for the prognosis of NSCLC. |
Key words: Prospero-related homeobox 1 non-small cell lung cancer epithelial-mesenchymal transition prognosis |