摘要: |
目的:探讨二甲双胍治疗坐骨神经慢性压迫损伤(chronic constriction injury,CCI)诱发大鼠神经病理性疼痛的效应。
方法:SPF级健康雌性SD大鼠48只,7~9 周龄,180~200 g,采用随机数字表法将其分为正常对照组(Naive组)、假手术组(Sham组)、CCI组和CCI+二甲双胍组(Met组),每组12只。二甲双胍采用灌胃方式给药,每次剂量45 mg/kg,从CCI建模前3 d开始给药,每天2次,持续至建模后第7 天。分别于建模前(基线水平),术后3 d、7 d、10 d、14 d记录建模侧(左侧)后足机械缩足反射阈值(MWT)、热缩足潜伏期(TWL)和冷刺激诱发缩足次数(TNCW);第3、14 天使用CatWalk步态分析系统测定各组大鼠的步态。
结果:与Naive组比较,CCI组和Met组大鼠左足MWT降低、TWL缩短、TNCW减少(P<0.05);CCI组大鼠左侧足印面积缩小、完整足印的平均强度减弱、站立时间缩短、步周长减小、摆动时间延长,并持续至建模后第14 天(P<0.05);Met组大鼠左侧足印面积缩小、完整足印的平均强度减弱;Sham组上述指标无明显改变。与CCI组相比,Met组大鼠左足MWT升高、TWL延长、TNCW增加(P<0.05); Met组大鼠左侧足印面积增大、完整足印的平均强度增强、站立时间延长以及步周长增加(P<0.05),摆动时间无明显改变。
结论:二甲双胍可缓解CCI诱发神经病理性疼痛大鼠机械痛觉过敏和冷热痛觉过敏,且能改善神经病理性疼痛大鼠的步态。 |
关键词: 神经病理性疼痛 慢性压迫损伤 二甲双胍 大鼠 |
DOI: |
分类号:R 977.1+5 |
基金项目:复旦大学附属中山医院青年基金(2016ZSQN62). |
|
Effect of metformin for treatment of neuropathic pain induced by sciatic nerve chronic constriction injury in rats |
WANG Zhi-yao, LI Wei-shan, LI Ai-lun, FANG Fang, CANG Jing*
|
Department of Anesthesiology, Zhongshan Hospital, Fudan University, Shanghai 200032, China
|
Abstract: |
Objective:To evaluate the effect of metformin for treatment of neuropathic pain induced by sciatic nerve chronic constriction injury in rats.
Methods:Forty-eight female SPF Sprague-Dawley rats (age, 7-9 weeks; weight, 180-200 g) were randomly divided into 4 groups (n=12 in each group): control group (Naive group), sham operation group (Sham group), CCI group and CCI + metformin group (Met group). Metformin (45 mg/kg, 2 times every day) was administrated orally from 3 d before to 7 d after operation. The left mechanical paw withdrawal threshold (MWT), thermal paw withdrawal latency (TWL), and number of cold stimulated paw withdrawal were measured before operation (baseline) and 3 d, 7 d, 10 d, 14 d after operation. CatWalk analysis system was used to measure the change in gait of rats in all groups 3 d and 14 d after operation.
Results:Compared with the Naive group, the left MWT, TWL, and TNCW decreased in the CCI and Met groups (P<0.05). Compared with Naive group, print area, mean print intensity, stand time, and stride length of left paw of rats decreased, but swing time increased in the CCI group, and continued to 14 d after operation (P<0.05); The print area, mean print intensity decreased in the Met group; above indexes in the Sham group were unchanged. Compared with the CCI group, the left MWT, TWL, and TNCW increased in Met group (P<0.05). Compared with the CCI group, the print area, mean print intensity, stand time, stride length of left paw of rats improved in the Met group (P<0.05), but the swing time was unchanged.
Conclusions:Metformin can alleviate mechanical hyperalgesia, thermal and cold allodynia in rats with CCI-induced neuropathic pain, and can improve the gait of rats with neuropathic pain. |
Key words: neuropathic pain chronic constriction injury metformin rats |